6H3D
Staphylopine dehydrogenase in complex with xNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.966 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 185.090, 48.750, 59.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.540 - 2.050 |
R-factor | 0.19344 |
Rwork | 0.192 |
R-free | 0.22852 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gmz |
RMSD bond length | 0.020 |
RMSD bond angle | 2.225 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.540 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 142598 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 99.2 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium acetate, 22% PEG 3350 |