6H3D
Staphylopine dehydrogenase in complex with xNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 185.090, 48.750, 59.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.540 - 2.050 |
| R-factor | 0.19344 |
| Rwork | 0.192 |
| R-free | 0.22852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gmz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.225 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.540 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 142598 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium acetate, 22% PEG 3350 |
