6H30
The crystal structure of SBD1-SBD2 tandem of GlnPQ transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.560, 187.033, 134.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.974 - 2.800 |
R-factor | 0.1676 |
Rwork | 0.164 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.063 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.140 | |
Number of reflections | 29326 | |
<I/σ(I)> | 4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 125 mM MES pH 6.0, 25 % PEG200, 6.25% PEG3350 and 50 mM NaF |