6H2F
Structure of the pre-pore AhlB of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.661, 178.184, 485.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.550 - 2.550 |
| R-factor | 0.19497 |
| Rwork | 0.191 |
| R-free | 0.27844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet AhlB |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.568 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.550 | 2.730 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.188 | |
| Rmeas | 0.751 | |
| Rpim | 0.122 | |
| Number of reflections | 165574 | 29762 |
| <I/σ(I)> | 6.6 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 0.2 M ammonium phosphate 0.1 M Tris pH 8.5 50 % (v/v) MPD |
