6H2E
Structure of the soluble AhlC of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9796 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 134.710, 134.710, 145.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.330 - 2.350 |
R-factor | 0.22874 |
Rwork | 0.227 |
R-free | 0.27364 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.534 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.330 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rpim | 0.023 | 0.639 |
Number of reflections | 32942 | 97395 |
<I/σ(I)> | 19.8 | 1.4 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 39.3 | 40.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.2 M Magnesium chloride 0.1 M Tris pH 7 10 % (w/v) PEG8000 |