6H2D
Structure of the soluble AhlC of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.070, 61.660, 129.770 |
| Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
| Resolution | 46.740 - 2.620 |
| R-factor | 0.27218 |
| Rwork | 0.269 |
| R-free | 0.33432 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet AhlC |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.475 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.740 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.074 | 0.710 |
| Rpim | 0.058 | 0.509 |
| Number of reflections | 31150 | 2309 |
| <I/σ(I)> | 11 | 1.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 0.2 M Sodium chloride 0.1 M Tris pH 8 20 % (w/v) PEG6000 |
