6H1P

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB - data collected at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2017-01-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	140.219, 140.219, 127.995
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.047 - 3.009
R-factor	0.184
Rwork	0.181
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.004
RMSD bond angle	0.777
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	44.050
High resolution limit [Å]	3.009	3.009
Rmeas	0.222
Number of reflections	29030
<I/σ(I)>	7.7
Completeness [%]	99.2
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	35% Tacsimate pH 8