6H19
Crystal structure of ethyl-paraoxon inhibited recombinant human bile salt activated lipase (aged form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-14 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.120, 97.810, 110.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.700 - 1.890 |
Rwork | 0.193 |
R-free | 0.22960 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 1.958 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.120 | |
Rmeas | 0.139 | |
Number of reflections | 49065 | 4813 |
<I/σ(I)> | 8.21 | 1.37 |
Completeness [%] | 99.3 | 98.75 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.995 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 8000, cacodylate, zinc acetate |