6H17
Myxococcus xanthus MglA bound to GTPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980073 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 117.349, 117.349, 117.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.910 - 1.275 |
| Rwork | 0.147 |
| R-free | 0.16270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 1.320 |
| High resolution limit [Å] | 1.275 | 1.275 |
| Rmerge | 0.062 | |
| Rmeas | 0.067 | |
| Rpim | 0.025 | 0.469 |
| Number of reflections | 67069 | 4993 |
| <I/σ(I)> | 14 | 1.43 |
| Completeness [%] | 96.0 | 63.23 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.999 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 277 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, 12% PEG 4000 |
