6H0R
X-ray structure of SRS2 fragment of Rgs4 3' UTR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.020, 32.390, 46.370 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 39.818 - 1.730 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23730 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.818 | 1.775 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.046 | 0.800 |
| Number of reflections | 16965 | 1218 |
| <I/σ(I)> | 17.9 | 2.07 |
| Completeness [%] | 96.7 | 94.8 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.999 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | sodium cacodylate, NaCl, BaCl2, MPD, spermine |
