6H0L
Hen egg-white lysozyme structure determined with data from the EuXFEL, 9.22 keV photon energy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
| Synchrotron site | European XFEL |
| Beamline | SPB/SFX |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | AGIPD |
| Wavelength(s) | 1.344 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.050, 80.050, 38.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.302 - 1.900 |
| R-factor | 0.1931 |
| Rwork | 0.188 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gf0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.054 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 18913 | |
| <I/σ(I)> | 6.3 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 278 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 3 | 293 | 20% NaCl, 6% PEG 6000 |
