6H0H
The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 in complex with beta-1,6-galactobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.350, 71.690, 88.710 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 30.211 - 1.390 |
| R-factor | 0.1176 |
| Rwork | 0.116 |
| R-free | 0.14640 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.927 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.211 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.045 | 0.393 |
| Rmeas | 0.052 | 0.499 |
| Rpim | 0.026 | 0.303 |
| Number of reflections | 130307 | 8129 |
| <I/σ(I)> | 18.59 | 2.39 |
| Completeness [%] | 91.8 | 57.69 |
| Redundancy | 3.8 | 2.4 |
| CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 10 % PEG 1000 10 % PEG 8000 |
