6H0A
Serum paraoxonase-1 by directed evolution with the L69G/H115W/H134R/F222S/T332S mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.93930 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 94.457, 94.457, 141.604 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.228 - 2.100 |
R-factor | 0.1887 |
Rwork | 0.188 |
R-free | 0.20550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.847 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.114 | 0.888 |
Rmeas | 0.118 | 0.920 |
Rpim | 0.031 | 0.238 |
Number of reflections | 38094 | 1853 |
<I/σ(I)> | 28.467 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.4 | 14.6 |
CC(1/2) | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris Propane pH 6.5, 0.2M Sodium Bromide and 20% PEG 3350 |