6H0A

Serum paraoxonase-1 by directed evolution with the L69G/H115W/H134R/F222S/T332S mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.93930
Spacegroup name	P 43 21 2
Unit cell lengths	94.457, 94.457, 141.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.228 - 2.100
R-factor	0.1887
Rwork	0.188
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.114	0.888
Rmeas	0.118	0.920
Rpim	0.031	0.238
Number of reflections	38094	1853
<I/σ(I)>	28.467	3
Completeness [%]	100.0	100
Redundancy	14.4	14.6
CC(1/2)		0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris Propane pH 6.5, 0.2M Sodium Bromide and 20% PEG 3350