6GZU
Structure of Chlamydia abortus effector protein ChlaDUB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.337, 41.775, 44.209 |
| Unit cell angles | 94.72, 102.17, 101.23 |
Refinement procedure
| Resolution | 42.855 - 1.470 |
| R-factor | 0.1532 |
| Rwork | 0.151 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hag |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.860 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.063 | 0.471 |
| Number of reflections | 45047 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 96.4 | 94.2 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris (pH 7.0), 0.2 M Calcium acetate, 20% PEG 3000 |
