6GZL
Complex between the dynein light chain DYNLL1/DLC8 and a peptide from the large myelin-associated glycoprotein L-MAG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-04 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 43.730, 43.730, 205.390 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.871 - 1.953 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zke |
RMSD bond length | 0.008 |
RMSD bond angle | 0.945 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.213 | 1.812 |
Rmeas | 0.222 | 1.901 |
Number of reflections | 6139 | 26 |
<I/σ(I)> | 13.34 | 1.3 |
Completeness [%] | 65.7 | 3.9 |
Redundancy | 13 | 11.4 |
CC(1/2) | 0.998 | 0.469 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate, and 25% (w/v) PEG 3350 |