6GZJ
Complex between the dynein light chain DYNLL1/DLC8 and the specific domain of large myelin-associated glycoprotein L-MAG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07175 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 44.050, 44.050, 204.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.148 - 1.977 |
R-factor | 0.1931 |
Rwork | 0.192 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zke |
RMSD bond length | 0.013 |
RMSD bond angle | 1.135 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.977 | 1.977 |
Rmerge | 0.107 | 0.813 |
Rmeas | 0.110 | 0.854 |
Number of reflections | 7535 | 62 |
<I/σ(I)> | 17 | 3.9 |
Completeness [%] | 82.8 | 9.7 |
Redundancy | 17.6 | 10.9 |
CC(1/2) | 0.999 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate, and 25% (w/v) PEG 3350 |