6GZC

heterotetrameric katanin p60:p80 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 1
Unit cell lengths	45.490, 57.850, 59.770
Unit cell angles	108.32, 101.00, 98.26

Refinement procedure

Resolution	43.575 - 2.000
R-factor	0.21
Rwork	0.209
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ow5
RMSD bond length	0.004
RMSD bond angle	0.538
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.600	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.041	1.534
Rmeas	0.045	1.656
Number of reflections	36685	2671
<I/σ(I)>	20.2	1.3
Completeness [%]	97.9	97
Redundancy	7	7
CC(1/2)	1.000	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	10% PEG 8000, 0.1 M HEPES (7.5), 8% EG