6GZA
Structure of murine leukemia virus capsid C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-08-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 71.198, 71.198, 77.068 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.599 - 1.890 |
R-factor | 0.1783 |
Rwork | 0.176 |
R-free | 0.21820 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.724 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.600 | 1.990 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 39681 | |
<I/σ(I)> | 18.9 | |
Completeness [%] | 100.0 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 37 mg/ml protein, 4% PEG 3350, 0.1 M HEPES pH 8.2, 5 mM CoCl2, 5 mM CdCl2, 5 mM MgCl2 and 5 mM NiCl2 |