6GYZ
Crystal structure of GlmM from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.653, 93.884, 156.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.482 - 3.000 |
R-factor | 0.2244 |
Rwork | 0.222 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pdk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.756 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.880 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.158 | 1.868 |
Number of reflections | 26871 | 4266 |
<I/σ(I)> | 8.4 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.5 | 12.5 |
CC(1/2) | 0.998 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2M Na-Malonate |