6GYE
Crystal structure of NadR protein in complex with NR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-30 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 165.140, 165.140, 196.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.363 - 2.300 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lw7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.958 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3150: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.370 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.160 | 2.980 |
| Number of reflections | 70447 | 8304 |
| <I/σ(I)> | 10.99 | 0.87 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1M (NH4)2SO4, 100mM Na Citrate, 10mM MgCl2 and 10% glucose |
