6GYB
Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.28 |
| Number of Particle | 185079 |
| Software | RELION,cryoSPARC,PHENIX,EPU,Gctf,Coot,UCSF Chimera |
| Symmetry | C14 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
