6GY3
Crystal Structure of C. glutamicum AmtR bound to glnA operator DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.320, 76.150, 117.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.290 - 2.680 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mqq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.289 | 2.750 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.072 | 0.618 |
Number of reflections | 16335 | |
<I/σ(I)> | 21.46 | 3.39 |
Completeness [%] | 98.8 | 90.1 |
Redundancy | 6.964 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M ammonium acetate, 0.1 M Tris-HCl, pH 8.5, 25% PEG 3350 |