6GX9

Crystal structure of the TNPO3 - CPSF6 RSLD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 63 2 2
Unit cell lengths	200.335, 200.335, 234.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.646 - 2.700
R-factor	0.2152
Rwork	0.214
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0o
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.690	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.171	1.931
Rmeas	0.179	2.018
Rpim	0.051	0.579
Number of reflections	76497	10987
<I/σ(I)>	11.6	1.5
Completeness [%]	100.0	100
Redundancy	12	12
CC(1/2)	0.998	0.422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	12.8% PEG MME 500, 6.4% PEG 20,000, 4% 1,6-hexandiol, 1.5% benzamidine, 75 mM NaCl, 40 mM MgCl2, 6 mM DTT, 80 mM Tris-bicine, pH 8.0