6GWZ
Blood group synthase AAGlyB in its apo form cryoprotected with PEG 3350
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.879, 149.625, 79.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.850 - 1.650 |
| R-factor | 0.16962 |
| Rwork | 0.168 |
| R-free | 0.20817 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3zgg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.631 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 38277 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MOPS buffer, Magnesium chloride, ammonium sulfate and PEG 3350 |
