6GW6

Structure of the Pseudomonas putida RES-Xre toxin-antitoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	60.060, 90.330, 186.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.165 - 2.205
R-factor	0.2159
Rwork	0.214
R-free	0.25290
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.165	2.284
High resolution limit [Å]	2.200	2.205
Number of reflections	51891
<I/σ(I)>	12.25
Completeness [%]	99.2
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Sodium malonate pH 6.0 and 20% w/v Polyethylene glycol 3350