6GW5
X-ray structure of the Helicobacter pylori SabA adhesin domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 69.146, 87.321, 169.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.500 - 2.520 |
| R-factor | 0.20477 |
| Rwork | 0.202 |
| R-free | 0.25565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5j |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.590 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmeas | 0.119 | 0.897 |
| Number of reflections | 140735 | 10599 |
| <I/σ(I)> | 11.8 | 2.03 |
| Completeness [%] | 97.0 | 98.1 |
| Redundancy | 4 | 4.2 |
| CC(1/2) | 0.997 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 15% PEG 3350, 0.2 M KF, 0.1 M citrate |
