6GVV
Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97166 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 93.120, 93.120, 121.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.600 - 2.350 |
R-factor | 0.22171 |
Rwork | 0.220 |
R-free | 0.26324 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u09 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.884 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC (rigid body refinement using as a model 1U09) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.084 | 0.426 |
Rpim | 0.035 | |
Number of reflections | 22406 | |
<I/σ(I)> | 11.4 | |
Completeness [%] | 98.1 | |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 4000, 0.2M magnesium acetate, 0.1M MES[2-(N-morpholino) ethanesulfonic acid] pH 6.0 and 4% a-butyrolactone. |