6GVM

Tubulin:F3II DARPin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	189.267, 51.721, 144.137
Unit cell angles	90.00, 121.03, 90.00

Refinement procedure

Resolution	49.280 - 3.500
R-factor	0.248
Rwork	0.245
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eyp 4f6r
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	3.730
High resolution limit [Å]	3.500	3.500
Number of reflections	15398
<I/σ(I)>	5.8
Completeness [%]	90.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG5000MME, Mes pH 6.0, 150 mM AMMONIUM SULFATE, 50 mM KCl