6GV0
Insulin glulisine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.440, 82.440, 33.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.486 - 1.260 |
| R-factor | 0.1292 |
| Rwork | 0.128 |
| R-free | 0.15180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ey9 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.887 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.220 | 1.280 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Number of reflections | 22986 | 3228 |
| <I/σ(I)> | 27 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2-0.4M Mg-formate 0.1M BisTris buffer |
