6GUX
Dark-adapted structure of Archaerhodopsin-3 at 100K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.701, 47.525, 104.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.290 - 1.300 |
R-factor | 0.1406 |
Rwork | 0.140 |
R-free | 0.17240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uaz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.301 |
Data reduction software | DIALS (1.10.1) |
Data scaling software | Aimless |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.740 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmeas | 0.201 | |
Rpim | 0.089 | |
Number of reflections | 66292 | 2345 |
<I/σ(I)> | 7 | 0.6 |
Completeness [%] | 94.5 | 68.8 |
Redundancy | 8.2 | 2.1 |
CC(1/2) | 0.994 | 0.057 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.5 | 293 | 100mM MES pH 5.5, 33% PEG 600, 150mM Na chloride, 150mM Ca chloride |