6GUA
Xylulose 5-phosphate phosphoketolase from Lactococcus lactis
This is a non-PDB format compatible entry.
Refinement Statistics
Experimental method: SOLUTION SCATTERING, X-RAY DIFFRACTION (1.95 Å)
| Cell axes | 158.100 | 141.500 | 161.150 |
| Cell angles | 90.00 | 90.29 | 90.00 |
| Spacegroup | P 1 21 1 | ||
| Resolution limits | 44.30 - 1.95 | ||
Crystallization Conditions
| crystal ID | method | pH | pH range | temperature | unit |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 |
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-21 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 158.100, 141.500, 161.150 |
| Unit cell angles | 90.00, 90.29, 90.00 |
Refinement procedure
| Resolution | 44.300 - 1.950 |
| Rwork | 0.212 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trk |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.152 | 0.913 |
| Rpim | 0.079 | 0.548 |
| Number of reflections | 501022 | 44418 |
| <I/σ(I)> | 6.79 | 1.27 |
| Completeness [%] | 97.3 | 86.6 |
| Redundancy | 3.5 | 2.5 |
| CC(1/2) | 0.995 | 0.183 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 17 % (w/v) PEG-3350, 0.2 M sodium isothiocyanate, |
