6GU1

SFI3 effector protein from the oomycete plant pathogen Phytophthora infestans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91
Spacegroup name	P 21 21 2
Unit cell lengths	43.080, 44.390, 62.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.050 - 1.700
R-factor	0.22108
Rwork	0.218
R-free	0.27431
RMSD bond length	0.014
RMSD bond angle	1.489
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	13594
<I/σ(I)>	22
Completeness [%]	99.5
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Bis-Tris Propane pH 7.5, 0.2 M sodium acetate, 20% (w/v) PEG 3350