6GTZ
Crystal structure of a FimH*DsG complex from E.coli F18 with bound dimannoside Man(alpha1-3)Man in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.637, 115.112, 63.158 |
| Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
| Resolution | 42.553 - 1.719 |
| R-factor | 0.1802 |
| Rwork | 0.179 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xoe |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.725 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.553 | 1.820 |
| High resolution limit [Å] | 1.719 | 1.719 |
| Rmeas | 0.084 | 0.732 |
| Number of reflections | 123705 | 6155 |
| <I/σ(I)> | 9.2 | 1.3 |
| Completeness [%] | 97.0 | 94.5 |
| Redundancy | 4.6 | 4.5 |
| CC(1/2) | 0.997 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M sodium cacodylate pH 6.5, 30% MPD |
