6GTV
Crystal structure of a FimH*DsG complex from E.coli F18 with bound trimannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00001 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 116.690, 119.210, 120.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.727 - 2.100 |
R-factor | 0.1892 |
Rwork | 0.188 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xoe |
RMSD bond length | 0.004 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.727 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.106 | 1.151 |
Number of reflections | 49075 | 7428 |
<I/σ(I)> | 13 | 1.8 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 6.6 | 6.4 |
CC(1/2) | 0.998 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M CaCl2, 0.1 M Hepes pH 7.5, 28% PEG 400 |