6GTS

Structure of the AtaT-AtaR complex bound DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	75.729, 87.920, 190.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.834 - 3.357
R-factor	0.2649
Rwork	0.264
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.725
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3161: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.240	3.640
High resolution limit [Å]	3.357	3.357
Rmerge	0.040	0.863
Number of reflections	7075
<I/σ(I)>	8.9
Completeness [%]	91.0
Redundancy	7.3
CC(1/2)		0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	0.1 M Bicine, 10%(w/v) PEG 20 000