6GTR
Structure of the AtaT Y144F mutant toxin bound to the C-terminus of the antitoxin AtaR and Acetyl-CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-07 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 87.880, 87.880, 125.610 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.540 - 2.990 |
R-factor | 0.196 |
Rwork | 0.193 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gtq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.540 | 3.110 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.085 | 0.300 |
Number of reflections | 11694 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1 M citrate, 20%(w/v) PEG 6000 |