6GT8
Sulfolobus solfataricus 2-keto-3-deoxygluconate aldolase Y132V,T157C variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-29 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 103.080, 103.080, 243.392 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.930 - 3.150 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w37 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.258 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.540 | 3.370 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.090 | 0.275 |
| Number of reflections | 13200 | |
| <I/σ(I)> | 22 | 5.3 |
| Completeness [%] | 95.2 | 82 |
| Redundancy | 12.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M HEPES pH 6.0, 15% PEG 4K, 8% isopropanol |
