6GT5
METTL16 MTase domain (crystal form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-02 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.550, 89.550, 179.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.100 - 2.450 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gfn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | XDS (BUILT 20171111) |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.100 | 2.800 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.360 | 1.580 |
Rmeas | 0.331 | 1.660 |
Rpim | 0.124 | 0.500 |
Number of reflections | 12391 | 619 |
<I/σ(I)> | 4.5 | 1.6 |
Completeness [%] | 93.3 | 79.9 |
Redundancy | 6.9 | 10.8 |
CC(1/2) | 0.967 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris propane, 0.2 M pottassium-sodium tartrate, 20% PEG 3350 |