6GT0
Nitrite-bound copper nitrite reductase from Achromobacter cycloclastes determined by serial femtosecond rotation crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-05 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.242 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 94.920, 94.920, 94.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.860 - 1.500 |
| R-factor | 0.14481 |
| Rwork | 0.143 |
| R-free | 0.17053 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.594 |
| Data reduction software | CrystFEL (0.6.3) |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.860 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 45846 | 2275 |
| <I/σ(I)> | 6.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 154.7 | 50.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 1.2 M ammonium sulphate, 100 mM citrate buffer, pH 5.0 |
