6GSZ
Crystal structure of native alfa-L-rhamnosidase from Aspergillus terreus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.260, 106.248, 119.832 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.820 - 1.380 |
R-factor | 0.1358 |
Rwork | 0.134 |
R-free | 0.16801 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.681 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.390 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmeas | 0.048 | 0.425 |
Number of reflections | 231137 | 4511 |
<I/σ(I)> | 31.5 | 1.1 |
Completeness [%] | 98.2 | 77.8 |
Redundancy | 3.9 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS pH 7.0, 40% PEG 300, 20 mM calcium acetate |