6GSL

Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	210.050, 449.390, 618.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.440 - 3.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	153.444	153.444	3.240
High resolution limit [Å]	3.160	14.130	3.160
Rmerge	0.384	0.056	8.012
Rmeas	0.386	0.057	8.060
Number of reflections	1936360	11745	72707
<I/σ(I)>	14.66	95.17	1
Completeness [%]	100.0	99.7	99.9
Redundancy	97.117	105.486	84.616
CC(1/2)	0.997	0.997	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Tris-OAc, KSCN, PEG-20000, PEG-550mme