6GSK
Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2399 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.820, 449.750, 618.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 224.875 - 3.360 |
R-factor | 0.2004 |
Rwork | 0.200 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v8d |
RMSD bond length | 0.009 |
RMSD bond angle | 1.490 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 224.875 | 224.875 | 3.450 |
High resolution limit [Å] | 3.360 | 15.030 | 3.360 |
Rmerge | 0.359 | 0.042 | 3.744 |
Rmeas | 0.364 | 0.042 | 3.826 |
Number of reflections | 1610361 | 17962 | 119307 |
<I/σ(I)> | 11.53 | 82.05 | 0.99 |
Completeness [%] | 99.9 | 99.9 | 100 |
Redundancy | 36.023 | 39.593 | 23.564 |
CC(1/2) | 0.998 | 0.998 | 0.316 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-OAc, KSCN, PEG-20000, PEG-550mme |