6GSD
Plantago Major multifunctional oxidoreductase in complex with progesterone and NADP+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-25 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.839, 78.839, 134.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.419 - 2.700 |
R-factor | 0.2082 |
Rwork | 0.205 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v6f |
RMSD bond length | 0.026 |
RMSD bond angle | 1.360 |
Data reduction software | XDS (June 1 2017) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.000 | 2.650 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmeas | 0.092 | |
Number of reflections | 13258 | |
<I/σ(I)> | 14.3 | 1 |
Completeness [%] | 95.0 | |
Redundancy | 7.6 | |
CC(1/2) | 0.910 | 0.270 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis Tris Propane, 5% glycerol, and 16.5% to 19% Peg 5K MME |