6GRZ
Crystal structure of the light chain dimer mH6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07227 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 79.887, 72.578, 84.836 |
| Unit cell angles | 90.00, 105.67, 90.00 |
Refinement procedure
| Resolution | 38.460 - 2.100 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mud |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.460 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | |
| Rmeas | 0.048 | |
| Rpim | 0.026 | 0.779 |
| Number of reflections | 27333 | 2214 |
| <I/σ(I)> | 13 | 0.8 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.02M Sodium Potassium Phosphate, 0.1M Bis-Tris Propane pH 8.5, 20% w/v PEG 3350 |
