6GRZ

Crystal structure of the light chain dimer mH6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07227
Spacegroup name	I 1 2 1
Unit cell lengths	79.887, 72.578, 84.836
Unit cell angles	90.00, 105.67, 90.00

Refinement procedure

Resolution	38.460 - 2.100
R-factor	0.208
Rwork	0.206
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mud
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.460	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.040
Rmeas	0.048
Rpim	0.026	0.779
Number of reflections	27333	2214
<I/σ(I)>	13	0.8
Completeness [%]	99.9	99
Redundancy	3.4
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.02M Sodium Potassium Phosphate, 0.1M Bis-Tris Propane pH 8.5, 20% w/v PEG 3350