6GRT

Paired immunoglobulin-like receptor B (PirB) or Leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3) full extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9686
Spacegroup name	P 1 21 1
Unit cell lengths	67.254, 127.103, 144.080
Unit cell angles	90.00, 103.43, 90.00

Refinement procedure

Resolution	70.070 - 4.504
R-factor	0.2612
Rwork	0.259
R-free	0.30710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d3v 4lla
RMSD bond length	0.002
RMSD bond angle	0.657
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.070	4.670
High resolution limit [Å]	4.500	4.500
Rmerge	0.226	0.907
Number of reflections	13858	1273
<I/σ(I)>	4
Completeness [%]	98.9	98
Redundancy	3.5	3.5
CC(1/2)	0.960	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	14.6% polyacrylate 5100 sodium salt, 0.07 M tris pH 8.0
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	14.6% polyacrylate 5100 sodium salt, 0.07 M tris pH 8.0