6GRQ

Paired immunoglobulin-like receptor B (PirB) or Leukocyte immunoglobulin-like receptor subfamily B member 3 (LILRB3) full extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 2 2
Unit cell lengths	106.375, 106.375, 217.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.188 - 3.300
R-factor	0.2502
Rwork	0.248
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d3v 4lla
RMSD bond length	0.003
RMSD bond angle	0.793
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.190	3.560
High resolution limit [Å]	3.300	3.300
Rmerge	0.277	2.767
Number of reflections	19634	3949
<I/σ(I)>	8.2	1.5
Completeness [%]	100.0	100
Redundancy	22.3	22.5
CC(1/2)	0.998	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	1.1 M LiCL, 14% PEG 6000, 0.1M citic acid buffer pH 5.5