6GRM

Structure of GFPmut2 crystallized at pH 6 and transferred to pH 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	31.323, 59.686, 108.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.126 - 2.300
R-factor	0.2283
Rwork	0.226
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q4a
RMSD bond length	0.008
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	Aimless (0.2.14)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.130	40.130	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.089	0.037	0.331
Rmeas	0.104	0.044	0.391
Rpim	0.053	0.024	0.202
Number of reflections	9288	181	913
<I/σ(I)>	7.9
Completeness [%]	97.9	89.5	99.1
Redundancy	3.3	2.9	3.3
CC(1/2)	0.994	0.996	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	MPD 45%