6GRJ
Structure of the AhlB pore of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97938 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 363.644, 116.526, 217.409 |
Unit cell angles | 90.00, 118.01, 90.00 |
Refinement procedure
Resolution | 102.670 - 2.940 |
R-factor | 0.22319 |
Rwork | 0.222 |
R-free | 0.23865 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.570 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.580 | 2.990 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 169188 | |
<I/σ(I)> | 1.127 | |
Completeness [%] | 99.0 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 60% Methyl-2,4-Pentanediol (MPD), 0.2M Ammonium phosphate, 0.1M Tris pH8.5 |