6GRF
Crystal structure of the tandem DUF26 ectodomain from the Arabidopsis thaliana cysteine-rich receptor-like protein PDLP8.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999871 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 143.862, 143.862, 59.722 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.100 - 1.950 |
R-factor | 0.23018 |
Rwork | 0.229 |
R-free | 0.26189 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gre |
RMSD bond length | 0.008 |
RMSD bond angle | 1.148 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.100 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmeas | 0.320 | 3.620 |
Number of reflections | 51859 | 8245 |
<I/σ(I)> | 8.82 | 1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20.3 | 19.7 |
CC(1/2) | 0.998 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 17.5 % polyethylene glycol 4,000, 0.1 M citrate pH 5.5, 20 % (v/v) 2-propanol |