6GRD
eukaryotic junction-resolving enzyme GEN-1 binding with Cesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.2 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 97.390, 97.390, 119.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 68.812 - 2.660 |
R-factor | 0.2454 |
Rwork | 0.244 |
R-free | 0.26240 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.307 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASES |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.812 | 2.730 |
High resolution limit [Å] | 2.660 | 2.660 |
Number of reflections | 19265 | |
<I/σ(I)> | 15 | |
Completeness [%] | 99.9 | |
Redundancy | 19.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 279 | PEG10000, NaCl, magnesium chloride, cesium chloride |