6GRC
eukaryotic junction-resolving enzyme GEN-1 binding with Sodium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91376 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.430, 98.430, 119.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.215 - 2.452 |
| R-factor | 0.2315 |
| Rwork | 0.230 |
| R-free | 0.25850 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.215 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.124 | 2.518 |
| Rmeas | 2.674 | |
| Rpim | 0.040 | 0.609 |
| Number of reflections | 25096 | 2405 |
| <I/σ(I)> | 16.3 | 1.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 20.1 | 18.8 |
| CC(1/2) | 0.999 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 279 | PEG10000 KCl magnesium chloride HEPES buffer pH7 |
